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Muristerone
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Sequoia
Sciences, Inc., a drug discovery company San Diego, CA aids high-throughput
screening (HTS) programs by providing novel libraries of chemically diverse
compounds that it isolates from plants. The unprecedented mass sensitivity of
Protasis microflow NMR probes coupled with the high-throughput, microfluidic
sample manager developed expressly for use with the flowprobes, now allows
Sequoia to acquire 1D proton and 2D Gradient COSY NMR spectra on quantities of
less than 10 micrograms in under ten minutes in its pursuit of rapid structure
elucidation. With the aid of the
ICG probe, Sequoia has been able to identify more than twenty new molecular
entities (NME’s) within the last nine months, a remarkable achievement in drug
discovery productivity. The two
prongs of Sequoia’s successful strategy are: (1) purification optimized by
multi-stage separations methods and (2) structure elucidation using micro-scale
NMR.
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Sample management is one of the most critical aspects of NMR analysis. Proper preparation of the sample enables a maximum amount of information to be extracted in minimum time, and accurate control of the experiment ensures data integrity, experimental reproducibility, and offers the option of subsequent (e.g. mass spectrometry) on-line analysis. The enhanced mass sensitivity, chromatographic resolution, and concentrating ability of capillary liquid chromatography enables the application scientist to take full advantage of the enhanced NMR mass sensitivity of MRM’s Microcoil™ probes.MRM chose to strategically align with Waters Corporation because we believe the quality of the Waters
CapLC® surpasses that of any similar instrument. Unlike
split-flow systems that waste valuable deuterated solvents, the CapLC
forms its solvent gradient directly with microdisplacers. And its
built-in photodiode array (PDA) UV/Vis detector includes a patented
Extended Path Length flow cell to provide full sensitivity in a
capillary detector flowcell. |
