| Protasis can also purify small molecules at high resolution as shown by this example. The example shows the enantiomer profile in the chamber resulted in a separation of 1.5 mg/ h of racemic after a 3 h batch run. The running buffer used was 1% Na-SCD, 10 mM Tris–acetate at pH 4.80 and the electrode buffer was 10 mM Tris–acetate at pH 4.80 adjusted to a conductivity of 3.0 mS with sodium chloride to match the conductivity of the running buffer. With the power supply set at 550 V (0.16 A/ cm ) and using a counterflow of 0.102 ml/ min, it was observed that the slow and fast enantiomers migrated in opposite directions within the chamber. This batch separation provided a solid starting point for a continuous separation by predicting an approximate counterflow rate and demonstrating that the enantiomer bands could, in fact, be forced to move in opposite directions by adjusting the counterflow. |
